Method
We developed PHYTOMap, a low-cost whole-mount method for multiplexed 3D RNA imaging in plants at single-molecule resolution. It enables spatial gene-expression analysis without tissue sectioning or generating a transgenic reporter for every target, and can be applied across diverse plant tissues and species. We have directly supported more than 10 research groups in implementing PHYTOMap, alongside independent applications of the method beyond our original experimental system.
Nature Plants · 2023
Nobori, T.*, Oliva, M., Lister, R. and Ecker, J.R.*
Nobori, T.*, Oliva, M., Lister, R. and Ecker, J.R.* (2023) Multiplexed single-cell 3D spatial gene expression analysis in plant tissue using PHYTOMap. Nature Plants. https://www.nature.com/articles/s41477-023-01439-4
Questions about PHYTOMap, or interested in working together? Write to Tatsuya.Nobori@tsl.ac.uk.